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Quality control (QC) samples were prepared by spiking fresh rat plasma with an appropriate dilution of dihexa for the final concentration of dihexa specified, keeping a 10: 1 ratio of plasma to dihexa solution (final DMSO concentration 5%)

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Key Takeaways Unreconstituted lyophilised IGF-1 LR3 lasts 2436 months at 20C with degradation rates below 2% per year when stored under continuous frozen conditions

We employed MR-Egger regression, weighted median, and modebased estimators (including both weighted and simple mode) to obtain robust causal effect estimates even if a subset of the instruments were invalid